UPLC-TUV同时测定双黄连口服液中3种有效成分

Simultaneous determination of 3 major components of Shuang-Huang-Lian Oral Liquid by UPLC-TUV

关键词:  超高效液相色谱  双黄连口服液  黄芩苷  绿原酸  连翘苷

摘要:

建立一种超高效液相色谱法同时测定双黄连口服液中黄芩苷、绿原酸、连翘苷的含量的方法。采用Acquity UPLC H-Class 超高效液相系统，Acquity UPLC TUV紫外检测器，BEH C18色谱柱 (2.1mm×100mm,1.7μm )，柱温25℃，流动相乙腈-0.1%甲酸梯度洗脱，流速0.3mL/min-1，进样体积4ul，检测波长278nm。结果表明，所检测的3种成分在测定的质量浓度范围内线性关系良好，r值均大于0.999，加样回收率在97%～103%之间，RSD均小于2.5%。试验精密度、重复性、准确性和稳定性良好。该方法简单、高效，灵敏度好，可用于双黄连口服液中3种成分的含量测定。

Abstract:

A detection method of baicalin and chlorogenicacid and forsythin in Shuanghuanglian oral liquid. The analysis was performed on a Waters BEH C18column ( 2.1 mm ×100 mm, 1.7μm) using acetonitrile-0. 1% formic acid as mobile phase, with a gradient elution at the flow rate of 0.30 mL/min. The column temperature was maintained at 25℃, and the detection wavelength was 278 nm by Acquity UPLC detector includes. The calibration curves were linear in certain ranges for three compounds (r＞0.999) respectively. The average recovery was 97%～103%and RSD were both less than 2.5%. This method was accurate and simultaneous analysis, which can be provided a reliable way for the quality control of Shuanghuanglian oral liquid.