皂树皂苷QS21增强猪支原体肺炎活疫苗滴鼻免疫小鼠效果评价

Adjuvant QS21 enhances immune responses in mice intranasally immunized Mycoplasma hyopneumoniae live vaccine

关键词:  QS21  佐剂  滴鼻  猪支原体肺炎活疫苗

Key words:  QS21  adjuvant  intranasal  Mycoplasma hyopneumoniae live vaccine

摘要:

为提高猪支原体肺炎活疫苗的滴鼻免疫效果,以猪支原体肺炎(168株)活疫苗为抗原,以QS21为免疫佐剂混合后滴鼻免疫小鼠,ELISA 法检测免疫效果,包括血清和支气管肺泡灌洗液中抗原特异性抗体水平,并测定了Th1型细胞因子IFN-γ、Th2型细胞因子IL-4和Th17型细胞因子IL-17的分泌情况。结果表明,QS21能激活黏膜和全身性的体液免疫,显著升高血清和支气管肺泡灌洗液中IgG,IgG1、IgG2a抗体含量,并能提高支气管肺泡灌洗液中黏膜免疫抗体sIgA的含量。QS21还能显著增加支气管肺泡灌洗液中IL-4、IFN-γ和IL-17的分泌,激活Th1、Th2和Th17型细胞免疫应答。以上结果表明QS21能全面激活免疫系统,具有开发为猪支原体肺炎活疫苗黏膜免疫佐剂的应用前景。

Abstract:

To improve intranasally immunized Mycoplasma hyopneumoniae live vaccine, this study used Mycoplasma hyopneumoniae live vaccine (168 strains) as antigen, mixed with QS21 as immune adjuvant, and then immunized mice by intranasally injection. ELISA method was used to detect immune effect, including the level of antigen-specific antibodies in serum and bronchoalveolar lavage fluid. The Th1 type cytokine IFN-γ, Th2 type cytokine IL-4 secretion and Th17 type cytokines IL-17 were also determined. The results showed that QS21 induced mucosal and systemic humoral immune response. QS21 increased the antibody level of immunized mice, promoted the production of IgG, IgG1, IgG2a antibodies in serum and bronchoalveolar lavage fluid, and increased the level of mucosal immune antibody sIgA. QS21 significantly increased the secretion of IL-4, IFN-γ and Th17 in bronchoalveolar lavage fluid and increased Th1, Th2 and Th17 type immune responses. The above results indicate that QS21 has great advantages in maintaining the balance of all types of immune responses and has the prospect of developing as a mucosal immune adjuvant for the Mycoplasma pneumonia live vaccine.