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鱼腥草芩蓝口服液的毒理学研究 |
喻琴,陈玲,尹伶灵,张世栋,董书伟,刘升,吕凤霞,那立冬,闫宝琪,杨洪早,王东升,严作廷 |
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(中国农业科学院兰州畜牧与兽药研究所) |
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摘要: |
为了评价鱼腥草芩蓝口服液的毒性,为临床安全用药提供依据。以昆明小鼠和SD大鼠为研究对象进行鱼腥草芩蓝口服液急性和长期毒性试验。急性毒性试验中,采用最大给药量试验,以健康小鼠20只,24 h内按68 g/kg体重灌胃给药3次,给药后连续观察10天,测定其最大给药量。长期毒性试验中,将80只健康大鼠随机分为对照组和高、中、低剂量组,每组雌雄各10只。对照组按20 mL/kg灌服生理盐水,给药组分别按34 g/kg、17 g/kg和8.5 g/kg灌服鱼腥草芩蓝口服液,每天一次,连续给药35 d。结果显示,小鼠灌服鱼腥草芩蓝口服液的最大给药量为204 g/kg。鱼腥草芩蓝口服液对试验大鼠的精神状态、脏器指数、生理生化指标均没有影响,且对主要脏器也没有明显的病理组织学损伤。表明鱼腥草芩蓝口服液在临床应用是安全的。 |
关键词: 鱼腥草芩蓝口服液 急性毒性试验 长期毒性试验 |
DOI: |
投稿时间:2018-02-01修订日期:2018-09-03 |
基金项目:“十三五”国家重点研发计划项目;中国农业科学院农业科技创新工程 |
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Toxicity of YuxingcaoQinlan oral liquid |
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(Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS,Key Laboratory of Veterinary Pharmaceutical Development of Ministry of Agriculture,Engineering Technology Research Center of Traditional Chinese Veterinary Medicine of Gansu Province,Gansu Lanzhou) |
Abstract: |
To evaluate the toxicity of YuxingcaoQinlan oral liquid and provide basis for clinical apply safety, the acute toxicity and long-term toxicity trial of YuxingcaoQinlan oral liquid were carried out. In the acute toxicity trial, 20 healthy mice were selected which were administered intragastrically with YuxingcaoQinlan oral of 68 g/kg.bw 3 times in 24 hours and continuously observed for 10 days. In the long-term toxicity test, 80 healthy rats were randomly divided into 4 groups that control group and high, middle and low group, 10 males and 10 females in each group in the trial. The Rat in the control groups were intragastric administrated with normal saline (20 mL/kg). The rat in YuxingcaoQinlan oral liquid-treated groups were treated with 34 g/kg, 17 g/kg and 8.5 g/kg.bw per day for 35 consecutive day, respectively. The results showed that the maximum daily dose of intragastric administration of YuxingcaoQinlan oral liquid in mice was 204 g/kg.bw. There were not affect activity, organ index and hematology and blood biochemical indicators and no histopathological changes in the tissues and organs of rats after administering YuxingcaoQinlan oral liquid. Our resultsindicated that administration of YuxingcaoQinlan oral liquid was safe in SD rats, suggesting potential safe use in clinic. |
Key words: YuxingcaoQinlan oral liquid acute toxicity trial long-term toxicity trial |