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高效液相色谱法测定板蓝根颗粒中腺苷的含量 |
王亚芳,钟昆芮,李应超,周艳飞,张小飞,高婷,秦俊杰,王海军,战皓 |
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(北京市兽药监察所;北京生泰尔生物科技有限公司) |
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摘要: |
建立了兽用板蓝根颗粒中腺苷含量测定的高效液相色谱法。以Symmetry C18柱为固定相,甲醇-0.05mol/L磷酸二氢钾溶液(10:90)为流动相,检测波长260nm,流速为1.2ml/min,柱温30℃。采用外标法定量计算含量。腺苷的线性范围为4~20μg/ml,回归方程y=71169x-6732,R2=0.9999;平均回收率96.62%,RSD为 1.08%。方法操作简单、精密度高、稳定性好,测定结果准确可靠,可用于板蓝根颗粒中腺苷的质量控制。 |
关键词: 腺苷 板蓝根颗粒 高效液相色谱法 质量控制 |
DOI: |
投稿时间:2017-12-21修订日期:2018-07-03 |
基金项目: |
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Determination of Adenosine in Banlangen Granules by HPLC |
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(Beijing Institute of Veterinary Drug Control) |
Abstract: |
A method was developed to determine the content of Adenosine in Banlangen Granules by HPLC, with Symmetry C18 column as stationary phase, mobile phase was methyl alcohol-0.05 mol/L potassium dihydrogen phosphate (10:90),detection wavelength was 260 nm, flow rate was 1.2 mL/min, and column temperature was 30 ℃. It was quantified by external standard method and adenosine in sample quantity is 4~20 μg/ml range, show a good linear relationship, y=71169x-6732(R2=0.9999). The recovery was 96.62%, RSD was 1.08%. This method is simple and accurate with good stability, it is suitable for determining the content of Adenosine in Banlangen Granules. |
Key words: Adenosine Banlangen Granules HPLC quality control |