小鹅瘟冻干卵黄抗体被动免疫持续期研究

The Maintenance Phase of Passive Immunity for frozen yolk antibody of gosling plague

关键词:  小鹅瘟冻干卵黄抗体  抗体效价  免疫持续期

摘要:

为探索小鹅瘟冻干卵黄抗体的被动免疫保护期及抗体效价与免疫持续期的平行相关性，试验采用3种琼扩效价分别为1∶16、1∶32和1∶64的实验室试制冻干卵黄抗体各3批，分别接种1日龄、4日龄健康易感雏鹅，1日龄雏鹅每只注射0.5 mL，4日龄雏鹅每只注射1.0 mL，并于注射后0 h、12 h、24 h、120 h、144 h、168 h、192 h、216 h对试验组和攻毒对照组接种小鹅瘟强毒W株，每只0.2 mL（含100 LD50）。结果显示：不同效价的卵黄抗体免疫后144 h内攻毒，卵黄抗体均可对雏鹅提供100%的免疫保护，雏鹅日龄越小，相对保护率越高，卵黄抗体琼扩效价越高，对雏鹅的免疫保护持续期越长。

Abstract:

In order to explore the passive immunological protection period and the parallel correlation between the antibody titer and immune duration of the gosling plague antibodies, three kinds of frozen yolk antibodies of gosling plague with antibody titers of 1∶16, 1∶32 and 1∶64 respectively, were inoculated to one-day-old and four-days-old healthy susceptible goslings with 0.5 mL and 1.0 mL, respectively. After the immunization of 0,12,24,120,144,168,192 and 216 hours, the experiment groups and the control groups were both infected by W strain of gosling plague virulent virus, 0.2 mL (including 100 LD50). The results showed that the egg yolk antibody could provide 100% immunity protection rate for goslings that were immunized yolk antibody of different titer, when gosling were inoculated gosling plague virus within 144 h. The smaller age goslings were, the higher the relative protection rate was; the higher the titer of the yolk antibody was, the longer the duration of immunization protection was.