离子色谱法测定盐酸头孢噻呋中的氯离子

Determination of Chloride Ion in Ceftiofur Hydrochloride by Ion Chromatography

关键词:  离子色谱  盐酸头孢噻呋  氯离子含量

Key words:  ion chromatography  ceftiofur hydrochloride  content of chloride

摘要:

建立了离子色谱法测定盐酸头孢噻呋中氯离子含量的方法。试验采用Dionex IonPac AS19（250 mm×4 mm，5 μm）阴离子交换色谱柱，以氢氧化钾溶液为淋洗液，流速为1.0 mL/min，柱温为30 ℃，检测池温度为35 ℃，抑制型电导检测器。试验结果表明氯离子与常见成盐阴离子分离良好，在0.5~20 μg/mL浓度范围内呈良好的线性关系（r2=0.9999），加样回收率为101.4%（n=9，RSD=1.7%）。本方法准确、灵敏、专属性好，可用于测定盐酸头孢噻呋中氯离子的含量，以控制成盐比例。

Abstract:

The method for content determination of chloride ion in ceftiofur hydrochloride by ion chromatography was developed. Analysis was performed on a Dionex IonPac AS19 column (250 mm×4 mm，5 μm).The elutriant was potassium hydroxide solution. The flow rate was 1.0 mL/min. The column temperature was set at 30 ℃. Inhibitory electrical conductivity detector was used with detecting pool temperature of 35 ℃. The results showed that a good separation was accepted between chloride ion and other conventional anions. The standard curve was linear in the range of 0.5~20 μg/mL(r2=0.9999). The average recovery was 101.4% (n=9，RSD=1.7%). This method was accurate，sensitive，specific and was suitable for the determination of chloride ion content in ceftiofur hydrochloride to control the salt-forming proportion.