小反刍兽疫活疫苗二次免疫效果评价

Evaluation of Secondary Immune Effects of Live Attenuated Peste des Petits Ruminants Vaccine

关键词:  绵羊  小反刍兽疫活疫苗  二次免疫  效果评价

Key words:  sheep  Live attenuated peste des petits ruminants vaccine  secondary immunization  Evaluation of secondary immune effects

摘要:

为研究已免疫小反刍兽疫（PPR）活疫苗的羊群经二次免疫后PPR抗体水平的变化趋势，对初免21 d羊群进行了第二次免疫，同时监测初免21 d及二免后3 d、7 d、14 d、21 d的血清中和抗体。结果显示，一次免疫羊21 d PPR中和抗体滴度平均可达5.18±2.87，阳性率为84%；二次免疫后3 d、7 d、14 d、21 d，PPR抗体呈上升趋势，平均滴度分别为5.65±2.56、6.95±1.82、7.28±1.18、8.12±1.31，阳性率分别为90%、100%、100%、100%。一免试验组21 d～323 d PPR抗体阳性率为83%，二免试验组在二次免疫7 d PPR抗体阳性率达到100%，并且维持到21 d。试验表明，活疫苗二次免疫增强了免疫效果，对已经有PPR抗体的羊进行二次免疫，可明显提升免疫抗体阳性率。

Abstract:

In order to research the antibody change tendency of secondary immunization, the sheep were injected with live attenuated peste des petits ruminants(PPR) vaccine at the 21st day after primary immunization. The PPR antibody at the 21st day after primary immunization and at the 3rd, 7th, 14th, 21st day after secondary immunization were detected respectively. The results indicated that the PPR antibody at 21st day after primary immunization reached to 5.18±2.87 and the positive rate was 84%. The PPR antibody level from the 3rd day to the 21st day after secondary immunization increased and the average titer were 5.65±2.56, 6.95±1.82, 7.28±1.18, 8.12±1.31 respectively, positive rate was 90%, 100%, 100%, 100% respectively. The PPR antibody positive rate at primary immunization group from 21days to 323days was 83%, PPR antibody positive rate reached to 100% at the 7th day after secondary immunization and kept high level to the 21st day, which showed that secondary immunization could enhance the effect of vaccine, and increase the positive rate of immune antibodies.