人参提取液体外抑制IHNV作用效果研究

Inhibition effect Ginseng extract on IHNV in vitro

关键词:  人参提取液,IHNV,抑制

Key words:  Panax  ginseng extract  liquid, IHNV, inhibition

摘要:

探究人参提取液体外抑制CCB细胞感染IHNV的能力，构建体外CCB细胞模型，运用RTCA技术和MTT法测得不同浓度人参提取液对IHNV抑制作用曲线，并从阻断病毒复制和杀灭病毒两方面讨论人参提取液作用效果。结果表明，4%人参提取液对IHNV具有明显抑制作用，能够在16h内保护细胞不受IHNV病毒的侵染，其中2h时对病毒灭活抑制率最高达到51.5%，1h-12h内对病毒复制有持续抑制作用。可为IHVN防治药物的研发提供新的思路及参考。

Abstract:

To delve the ability of Panax ginseng extractliquid inhibition IHNV infected CCB cells, CCB cell model was constructed in vitro,using RTCA technology and MTT method the inhibitory action curve of ginseng extract on IHNV was measured, and discussing the effect of ginseng extracliquid from blocking virus replication and killing two aspects of the virus. The results showed that 4% ginseng extract liquidhad obvious inhibitory effect on IHNV，it can protect cells from infection by IHNV virus for a certain period of time, When 2h, the inhibition rate of virus inactivation was highest, reaching 51.5%, 1h-12h has a continuous inhibitory effect on viral replication.IHVN prevention and treatment of drug research and development to provide new ideas and reference.