| 摘要: |
| 为了探讨致病性大肠杆菌内HMWP2蛋白的生物学结构和功能，本研究采用Red同源重组技术，对致病性E.coli出发株的HPI irp2基因进行敲除，成功构建了致病性E.coli irp2基因缺失株，并利用测序结果对HMWP2蛋白进行了多重预测分析，以期为致病性大肠杆菌相关疾病的防治和发生机理研究提供理论基础。 |
| 关键词: 致病性E.coli irp2基因 HMWP2 Red同源重组 基因敲除 |
| DOI： |
| 投稿时间：2017-05-31修订日期：2018-01-10 |
| 基金项目:国家自然科学基金项目（31260594；31660704） |
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| Construction of PEC HPI irp2 Gene Deletion Strains and Structure and Function Prediction of HMWP2 |
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| (Faculty of Animal Science and Technology) |
| Abstract: |
| In order to investigate the biological structure and function of HMWP2 protein in PEC,the irp2 gene was knocked down by Red homologous recombination technique and irp2 gene deletion strain was successfully constructed, then the structure and function of HMWP2 protein was predicted and analysised by irp2’s sequence. This study aims to provide a theoretical basis for prevention and pathogenesis of E. coli-related diseases. |
| Key words: PEC irp2 gene HMWP2 Red homologous recombination Gene knock-out |
