BHK-21细胞不同处理方式对裸鼠致瘤性的影响

Effects of Different Treatments of BHK-21 Cells on Tumorigenicity in Nude Mice

关键词:  BHK-21细胞  裸鼠  致瘤性

Key words:  BHK-21 cells  nude mice  tumorigenicity  

摘要:

为了分析BHK-21细胞对裸鼠致瘤性受何种因素的影响,我们分别用甲醛、二乙烯亚胺(BEI)、β-丙内酯(BPL)进行灭活和在-20℃进行冻融处理的BHK-21细胞接种裸鼠,通过病理组织形态学观察判定其是否存在致肿瘤的特性。结果显示,甲醛、BEI、BPL、冻融2次或3次分别处理的BHK-21细胞接种裸鼠,对裸鼠致瘤率为零。冻融1次的BHK-21细胞和107、105和103个BHK-21细胞分别接种裸鼠,致瘤率为100%。10个BHK-21细胞接种裸鼠,未见成瘤。本次试验表明,裸鼠检测BHK-21细胞致瘤性敏感性为103个细胞；冻融2次、甲醛、BEI、BPL处理,BHK-21细胞均丧失致瘤性；冻融1次不能使BHK-21细胞丧失致瘤性。

Abstract:

For understanding the tumorigenicity of BHK-21 cells, nude mice were injected by BHK-21 cells which inactivated by formaldehyde, diethylenimine (BEI), β-propiolactone (BPL) as well as BHK-21 cells which freezing-thawing at -20℃, the tumor-bearing characteristics were observed by pathomorphological?changes. The results showed that the tumor morbidity of BHK-21 cells were treated by formaldehyde, BEI, BPL and freezing-thawing for two or three times were zero, 107, 105 , 103 BHK-21 cells can 100% cause the tumors as well as the BHK-21 cells were freezing-thawing only once, no tumors were found when the dose of injection is ten. These results suggested that the sensitivity of tumorigenicity of BHK-21 cells in nude mice was 103 cells, the tumorigenicity of BHK-21 cells can be killed with freezing-thawing for twice, formaldehyde, BEI and BPL but it still have tumorigenicity in nude mice with freezing-thawing only once.