水产养殖用复合微生物粉剂中菌种的分子生物学鉴定

Molecular Identification of strains isolated from composite microbial powders for aquaculture

关键词:  水产养殖  复合微生物粉剂  菌群结构  鉴定  16S rDNA  26S rDNA

Key words:  aquaculture, composite microbial powder, microflora structure, identification, 16S rDNA, 26S rDNA

摘要:

对两种市售水产养殖用复合微生物粉剂中微生物菌种进行鉴定。利用平板分离技术对粉剂中的微生物进行分离纯化,进行了形态学观察、16S rDNA和26S rDNA序列分析,并构建系统发育树。结果表明,两种微生物粉剂中菌群结构存在一定差异,S1粉剂中鉴定出5株芽孢杆菌和2株酵母菌,分别为枯草芽孢杆菌、地衣芽孢杆菌或索诺拉沙漠芽孢杆菌、葡萄牙棒孢酵母和库德里阿兹威毕赤酵母；S2粉剂中鉴定出3株芽孢杆菌、1株放线菌、2株酵母菌和3株肠球菌,分别为解木糖赖氨酸芽孢杆菌、环状芽孢杆菌、地衣芽孢杆菌或索诺拉沙漠芽孢杆菌、芬氏纤维微菌、酿酒酵母、库德里阿兹威毕赤酵母、鹑鸡肠球菌、屎肠球菌或乳酸肠球菌。明确水产养殖用复合微生物粉剂的菌群结构为同类产品的安全性及有效性评估提供理论基础。

Abstract:

In order to identify microbial strains in two commercial composite microbial powders for aquaculture, plate separation method was used to isolate and purify the microorganisms, morphological characteristics was observed, 16S rDNA/26S rDNA gene sequences analysis was conducted and phylogenetic trees were constructed. Variation in microflora structure existed between two microbial powders. Five Bacillus strains and two yeast strains were identified in S1 powder, including Bacillus subtilis, Bacillus licheniformis or Bacillus sonorensis，Clavispora lusitaniae and Pichia kudriavzevii; Three Bacillus strains, one Actinomycete strain, two yeast strains and three Enterococcus were identified in S2 powder, including Lysinibacillus xylanilyticus, Bacillus circulans, Bacillus licheniformis or Bacillus sonorensis, Cellulosimicrobium funkei, Saccharomyces cerevisiae, Pichia kudriavzevii, Enterococcus gallinarum, Enterococcus faecium or Enterococcus lactis. Determination of the microflora structure of composite microbial powders for aquaculture would provide theoretical basis for evaluation of safety and effectiveness of similar products.