不同诱变方法对TL-15028菌株产泰乐菌素相关性能的影响

The Effect of Different Induction Mutation Method on Production Performance for Tylosin in TL-15028 Strain

关键词:  泰乐菌素  高产菌株  复合诱变  选育

Key words:  tylosin  high yield strain  compound mutation  screening

摘要:

为了选育出泰乐菌素高产菌株，以弗氏链霉菌 (Streptomyces fradiae) TL-15028菌株作为出发菌株，利用化学诱变、物理诱变以及复合诱变的方法对菌株进行诱变处理，比较不同诱变方法的诱变效果，通过诱变筛选出1株遗传稳定性好、且具有泰乐菌素和豆油抗性的菌株TLEU-1503，其发酵效价比出发菌株提高了36.5%，达到14124 μg/mL，对泰乐菌素高效生产、品质提升具有重要意义。

Abstract:

In order to screening of high yield strain for tylosin, TL-15028 strain was selected as original strain. The original strain was mutation treated with chemical mutation, physical mutation and compound mutation, respectively. The mutagenic effect of the three methods were compared. The TLEU-1503 strain, which with high inheritance stability and resistance to tylosin and soybean oil, was screened. The potency of TLEU-1503 strain, which was 14124 μg/mL, had improved 36.5% than original strain. The result had great significance for efficient production and quality improvement of tylosin.