三种中药提取物体外抗猪繁殖与呼吸综合征病毒的作用

Antiviral Action of 3 Kind of Traditional Chinese Medicine Extract against Porcine Reproductive and Respiratory Syndrome Virus in vitro

关键词:  中药提取成分  抗病毒  PRRSV

Key words:  extract of Chinese medicinal herbs  antiviral action  PRRSV

摘要:

在前期筛选具有抗猪繁殖与呼吸综合征病毒(PRRSV)作用的中药研究基础上，选取药效较好的三种中药：穿心莲、芦根和夏枯草。为进一步研究其有效抗病毒成分，采用MTT 法观察了穿心莲内酯盐、芦根多糖和夏枯草多糖成分对猴肾上皮细胞系(Marc-145) 生长的影响，分别采用阻断、抑制、直接杀灭3种作用方式观察了各成分抗RPPSV感染作用。结果显示：穿心莲内酯盐对细胞最大安全浓度为0.16 mg/mL，芦根多糖为0.575 mg/mL，夏枯草多糖为1.125 mg/mL；芦根多糖在三种方式上均有不同程度的抗PRRSV作用，而穿心莲内酯盐和夏枯草多糖阻断PRRSV作用不明显，抑制和杀灭PRRSV作用明显。结果表明穿心莲内酯盐、芦根多糖和夏枯草多糖均有抗病毒作用，为抗病毒中药的主要药效成分。

Abstract:

Andrographolide, phragmites polysaccharide and selfheal polysaccharide were selected to study the antiviral action against to porcine reproductive and respiratory syndrome virus(PRRSV) in vitro. The blocking, inhibiting and killing roles of these extract were detected in Marc-145 cells infected with PRRSV. Results showed the safety concentration of andrographolide, phragmites polysaccharide and selfheal polysaccharide respectively are: 0.16, 0.575 and 1.125 mg/mL. All the 3 extracts had antiviral actions against to PPRSV by inhibiting and killing roles，but just phragmites polysaccharide has the blocking role in highest safety concentration. And the way of extract acting in protection of the cell was as the same as the whole herb, indicate these extract are the major portion of the Chinese medicine herb.