UPLC-MS/MS法测定氟喹诺酮类药物可溶性粉中非法添加物利巴韦林

Determination of Ribavirin Illegally Added in the FQs soluble powder by UPLC-MS/MS

关键词:  利巴韦林  氟喹诺酮类药物  超高效液相-串联质谱法

Key words:  Ribavirin  FQs Veterinary Medicine  UPLC-MS/MS

摘要:

建立了氟喹诺酮类药物可溶性粉中非法添加物利巴韦林检测的UPLC-MS/MS方法。样品经提取稀释后，采用ACQUITY UPLCR BEH amide色谱柱为分离柱，质谱正离子扫描测定。结果显示，利巴韦林在1~50 ng/mL的范围内线性关系良好（R2=0.9984）；样品检出限为0.5 mg/g，定量限为2 mg/g。在40、50、60mg/g添加水平的回收率为90%～110%，批内批间变异系数均小于10%。本方法快速、灵敏、重现性好，适用于氟喹诺酮类药物可溶性粉中非法添加利巴韦林的检测。

Abstract:

A method was established for determination of ribavirin added in FQs soluble powder by UPLC-MS/MS. The ribavirin were extracted from diluted samples and separated by ACQUITY UPLCR BEH amide. The ribavirin was analyzed by UPLC-MS/MS with positive ion mode.The results showed that the good linear range was 1 to 50 ng/mL（R2=0.9984）. The detection limit was 0.5mg/g，and the quantitation limit was 2 mg/g. The analysis of added 40、50、60mg/g ribavirin showed that the recoveries ranged from 90% to 110% and the relative standard deviations were below 10%. The method was speedy, sensitive and reproducibility，suitable for the determination of ribavirin added in FQs soluble powder .