兽用鸡脾转移因子的多肽组成研究

The Diverse Polypeptides Consisting of the Chicken Spleen Transfer Factor in Veterinary

关键词:  转移因子  多肽  液相色谱-质谱分析

Key words:  transfer factor  polypeptide  LC-MS

摘要:

为了研究兽用鸡脾转移因子的多肽组成，利用冻融-透析法制备兽用鸡脾转移因子溶液，分别采用福林酚法测定多肽含量，基质辅助激光解析电离飞行时间质谱法（MALDI-TOF-MS）测定分子量，液相色谱-质谱联用法（LC-MS）分析多肽组成，脱E受体法测定转移因子活力。鸡脾转移因子溶液多肽含量为1570.8 μg/mL，活力为10.92%，LC-MS法测得多肽分子量为800～7476 Da，多肽种类581种，包括T细胞凋亡抑制相关蛋白（TIAL1）等多种生物活性蛋白。初步揭示了兽用鸡脾转移因子的多肽组成，有助于进一步研究其有效成分和作用机理。

Abstract:

To make clear of the diverse polypeptides composition consisting of the chicken spleen transfer factor in veterinary. Firstly, freeze thawing method was used in making transfer factor solution from chicken spleen. Secondly, the peptide content and composition, molecular weight, and receptor activity were determined by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), liquid chromatography - mass spectrometry (LC-MS) and E rosette method, respectively. The preparation of chicken spleen transfer factor solution polypeptide was 1570.8 μg/mL, activity was 10.92%, the molecular weight of the peptide LC-MS were ranged around 800-7476 Da. There were 581 kinds of polypeptide species, including T inhibitor of apoptosis related protein (TIAL1) and other biologically active protein. The results of the transfer factor composition from the chicken spleen would help us to discover more active ingredients and mechanism in veterinary, and more research works are warranted to be finished in future.