鸡传染性鼻炎灭活疫苗生产检验用副鸡禽杆菌Hpg-8株的保存期试验

Preservation Period Test of the Hpg-8 Strain of A. paragallinarum Master Seed of Infectious Croyza inactivated Vaccine

关键词:  副鸡禽杆菌  毒力  免疫原性  保存期

Key words:  A. paragallinarum  virullence  immunogenicity  preservation period

摘要:

本研究对不同冻干年代的副鸡禽杆菌Hpg-8株菌种分别进行了毒力测定和免疫原性试验。分别用1991、1997、2001、2011年冻干的菌种，以约3万CFU/羽眶下窦接种2月龄SPF母鸡，结果均可使鸡5/5发病；分别用1991、1997、2011年冻干的菌种，经繁殖制备疫苗后接种SPF鸡，结果可提供8/8、7/7、7/7保护。以上结果表明，副鸡禽杆菌Hpg-8株在－70℃保存10、14、20年后的毒力和在－70℃保存14、20年后的免疫原性仍符合《中华人民共和国兽用生物制品规程》（二○○○版）的规定。

Abstract:

The virulence on SPF chicken and immunogenicity of the Hpg-8 Strain cultures of A. paragallinarum manufactured at different years were tested repectively. 5/5 chicken showed the clinical symptoms of infectious coryza after intrasinus injected respectively with about 30,000CFU of the Hpg-8 Strain cultures of A. paragallinarum producted at 1991, 1997, 2001 and 2011. The protection rates of 8/8, 7/7 and 7/7 were obtained after chickens were inoculated with the coryza vaccine with the Hpg-8 Strain cultures of A. paragallinarum producted at 1991, 1997, and 2011. The results indicated that the virulence on SPF chicken of the Hpg-8 Strain cultures of A. paragallinarum stored at －70℃ for 10, 14, 20 years and the immunogenicity of the Hpg-8 Strain cultures of A. paragallinarum stored at －70℃ for 14, 20 years still conformed to the regulations of The People’s Republic of China Veterinary Biological Products(2000 edition).