| 摘要: |
| 采用液相色谱-串联质谱法测定动物尿液中11种β-受体激动剂残留量,对标准溶液、体积、质谱峰面积、浓缩过程及回收率等测定不确定度因素进行了分析,通过评定各不确定度分量及标准不确定度,得出11种β-受体激动剂的扩展不确定度在0.7～1.1 ng/mL范围内。由各因素对合成不确定度的贡献比分析可知,影响较大的因素为试验回收率及标准溶液浓度。 |
| 关键词: 不确定度 β-受体激动剂 液相色谱串联质谱 评估 |
| DOI： |
| 投稿时间：2013-01-05修订日期：2013-05-09 |
| 基金项目: |
|
| ZHOU Wei, YING Yong-fei, WEI Ming-jue, LU Chun-bo, LIN Xian-jun, LUO Cheng-jiang, ZHANG Zhi-jian, ZHU Cong-ying1 |
|
| (Institute of Veterinary Drug Control of Zhejiang Province;China) |
| Abstract: |
| Evaluation on uncertainty of measurement in the determination of 11 kinds of b-receptor agonists in animal urine by liquid chromatography-tandem mass spectrometry (LC-MS/MS) was practiced. The main factors, include standard solution, volume, mass chromatographic peak area, enrichment process and recovery were analyzed. The combined uncertainty was finally obtained by synthesizing the uncertainties of various components. The expanded uncertainty of 11 kinds of b-receptor agonists was in the range of 0.7～1.1 ng?mL-1. According to the contribution of all components, the key ones that affect the combined uncertainty were the concentrations of standards and the recoveries. |
| Key words: uncertainty b-receptor agonists LC-MS/MS evaluation |
