C18-磁性纳米颗粒萃取虾肉中氯霉素残留

Application of C18-magnetic Nano-particles in Pre-treatment of Chloramphenicol Residues Detection of Shrimp

关键词:  固相萃取；C18-磁珠  氯霉素

Key words:  solid phase extraction  C18-functionalized magnetic silica nanoparticles  chloramphenicol

摘要:

建立了一种用C18键合磁珠固相萃取虾肉中氯霉素残留的方法。在Fe3O4超顺磁性纳米颗粒的表面键合C18基团，制备双功能反相萃取颗粒。用该颗粒对样品中的氯霉素残留进行固相萃取，对结合时间、温度、磁珠用量等因素进行了优化，建立磁珠法兽药残留固相萃取方法。用标准方法（SN/T 1864-2007）对萃取所得产物进行检测。经过优化，C18键合磁珠的最佳萃取条件为50℃结合5min，用1mL甲醇洗脱3次。检测结果显示该方法具有良好的精密度和回收率。在0.1~10μg/kg之间具有良好的线性关系，相关系数为0.9941，检出限为0.1μg/kg。结论:实验结果表明,该方法灵敏度高、重现性好、准确度高,可满足肉类中氯霉素残留检测的需要。

Abstract:

Objective：A method of C18-functionalized magnetic silica nanoparticles solid phase extraction chloramphenicol residues in meat was developed. Method：The reversed-phase extraction dual particles were synthetized by bonding C18 groups on the surface of the superparamagnetic Fe3O4 nanoparticles and were used to extract veterinary drug residues by SPE.The process conditions, such as temperature, uniting time and additive amount of C18-functionalized magnetic silica nanoparticles were optimized. Results：The detection method showed good linear relationship in the range of 0.1ug/kg～10ug/kg(r=0.9941) and the detection limit is 0.1ug/kg. The recovery ranges were between 73.3%~112.3%. Conclusion:The method is reliable,accurate and has a good reproducibility which can meet the demands of the detection of chloramphenicol residues in meat.