链霉素抗性突变选育那西肽高产菌株

Breeding of High-producing Nosiheptide Strain by Streptomycin Resistance Mutation

关键词:  链霉素抗性  诱变育种  那西肽  稳定

Key words:  streptomycin resistance  mutation breeding  nosiheptide  stable

摘要:

通过对那西肽产生菌N106的孢子进行紫外诱变处理（15 W，253.7 nm，距离30 cm），再利用链霉素抗性突变作为筛选模型（链霉素最低抑制浓度为3 u/mL）获得了链霉素抗性基因突变的高产菌株NUS146和NUS182，摇瓶发酵水平分别达到3233和3452 u/mL，比出发菌株N106的摇瓶水平2502 u/mL分别提高了29.22%和37.97%。传代试验表明，该两突变株遗传特性相对稳定。

Abstract:

The spores of nosiheptide producing strain N106 were mutated with UV light (15W, 253.7 nm, Distance of 30 cm), and the streptomycin (its minimum lethal dose was 3 u/mL) resistance mutation was used as the screen model to obtain the streptomycin-resistant mutant. Consequently two high-producing strains of NUS146 and NUS182 were got. Compared the activity of shaking flask fermentation, the high-yield strains could reach 3233 and 3452 u/mL which increased by 29.22% and 37.97% respectively, while the strain N106 was only 2502 u/mL. The generation test results showed that the genetic characters of two mutants were relatively stable.