| 摘要: |
| 为评价猪口蹄疫合成肽疫苗免疫猪后的细胞免疫应答，用含有E、F两种多肽抗原的猪口蹄疫合成肽疫苗免疫猪，二免后两周采血分离猪外周血淋巴细胞，再用E、F两种合成肽及二者混合物对淋巴细胞刺激培养48 h，用MTT法检测特异性T淋巴细胞增殖反应。结果显示，在抗原E、F混合物浓度为50 μg/mL时，抗原对免疫组淋巴结细胞的刺激增殖作用显著高于未免疫对照组（P＜0.01）。表明，该猪口蹄疫合成肽疫苗免疫猪能有效引起特异性T淋巴细胞免疫应答。 |
| 关键词: MTT法；口蹄疫；合成肽疫苗 T淋巴细胞增殖反应 |
| DOI： |
| 投稿时间：2010-08-19修订日期：2011-01-12 |
| 基金项目: |
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| Cellullar Immunologic Response of Immunized Swine Following Synthetical Peptide Vaccine by MTT Assay |
| qipeng |
| (China Animal Husbandry Industry Company Limited) |
| Abstract: |
| In order to observe the level of cellular immunity, swines were immunized with synthetical peptide vaccine and T lymphocyte proliferate response was investigeted by MTT assay. The synthetical peptide vaccine contained two different peptides E and F. The swine peripheral blood T lymphocyte was collected after the second immunization, and T lymphocyte was cultivated with peptide E、peptide F and their mixture respectively for 48 h. Compared with the control group, the mixtrue with concentration of 50 μg/mL of immunizing group can increase T lymphocyte proliferation and stimulate swine cellular immune response significantly. This record indicated the swine foot and mouth disease synthetical peptide vaccine can stimulate T lymphocyte proliferate response significantly. |
| Key words: MTT Assay foot and mouth disease Synthetical Peptide Vaccine T Lymphocyte Prolifeate Response |
