HPLC测定苦参注射液中苦参碱的含量

Content Determination of Matrine in Matrine Injection by HPLC

关键词:  高效液相色谱法  苦参注射液  苦参碱

Key words:  HPLC  matrine injection  matrine

摘要:

建立了苦参注射液中苦参碱含量的HPLC检测法。色谱条件为：色谱柱waters C18（4.6mm×150mm，5μm）；以0.02mol/L乙酸铵缓冲溶液（含500μL/L三乙胺）-甲醇-乙腈（70:10:20）为流动相；检测波长210nm；流速为1mL/min；柱温30℃；进样量20μL。苦参碱进样浓度在4.0～200.0μg/mL的范围内与峰面积呈良好的线性关系（r＝0.9999）；样品平均回收率为100.1%（n＝9），RSD为1.1%。本方法简便、准确度高、重复性好，可用于苦参注射液中苦参碱的质量控制。

Abstract:

Abstrac: An HPLC method for the content determination of matrine in matrine injection was developed . The Column was Waters C18 (4.6 mm×150 mm,5μm) , mobile phase was 0.02mol/L acetate buffer solution (500μL/L triethylamine) -methanol-acetonitrile (70:10:20), detection wavelength was 210 nm, flow rate was 1 mL /min. The column temperature was 30℃. The injection volume was 20μL.There was a good linear correlation between the peak areas and the concentrations of matrine at the range of 4.0～200.0μg/mL (r=0.9999). The average recovery was 100.1％with RSD of 1.1％(n=9)．The results indicated that the method was simple, accurate and repeatable. It can be used for the quality control of matrine in matrine injection.