| 摘要: |
| 将重组质粒pGEM-T-SOD中的副结核分枝杆菌SOD基因亚克隆至真核表达载体pVAX1中，构建真核表达重组质粒pVAX1-SOD，以脂质体介导法转染至BHK-21细胞中，并采用RT-PCR和间接免疫荧光技术检测SOD基因在BHK-21细胞中的表达。结果显示，成功地构建了副结核分枝杆菌SOD基因的真核表达载体，且SOD基因在哺乳动物细胞中获得了表达。为研究副结核分枝杆菌SOD基因的免疫效果及作为DNA疫苗奠定基础。 |
| 关键词: 副结核分枝杆菌 SOD基因 真核表达 |
| DOI： |
| 投稿时间：2010-04-04修订日期：2010-05-25 |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
|
| Expression of Mycobacterium paratuberculosis SOD Gene in BHK-21 Cells |
| huyuqing |
| (Jilin Agricultural University) |
| Abstract: |
| Mycobacterium paratuberculosis SOD gene of the recombinant plasmid pGEM-T-SOD was subcloned into eukaryotic expression vector pVAX1 and construct the recombinant plasmid pVAX1-SOD. Transfect BHK-21 cells with the constructed recombinant plasmid in mediation of liposome, and observe the expression of SOD gene in BHK-21 cells by RT-PCR and indirect immunofluorescence technique.The results show that the eukaryotic expression vector for Mycobacterium paratuberculosis SOD gene was successfully constructed and expressed in mammalian cells,which laid a foundation of developing immune effect of Mycobacterium paratuberculosis SOD gene and DNA vaccine for Bovine Paratuberculosis. |
| Key words: Mycobacterium paratuberculosis SOD gene Eukaryotic express |
