| 摘要: |
| 摘 要：目的 建立HPLC法同时测定药物氟尼康中盐酸多西环素、氟苯尼考的含量。方法 采用Hypersil-ODS2 （250 mm×4.6 mm,5 μm）色谱柱，流动相为乙腈：甲醇：磷酸二氢铵（0.1 mol•L-1）=1:2:5，pH=3.50，检测波长为254 nm，流速为1 mL•min-1。结果 实现了氟尼康中盐酸多西环素、氟苯尼考的较好分离；盐酸多西环素在6.25 μg•mL-1~200 μg•mL-1范围内线性关系良好，r=0.9994,平均回收率为99.29%，RSD为0.51%；氟苯尼考在6.25 μg•mL-1~250 μg•mL-1范围内线性关系良好，r=0.9999，平均回收率为98.87% ，RSD为0.32%。结论 该方法快速简便，灵敏度高，重现性好，可用于药物氟尼康质量控制的测定方法 |
| 关键词: 高效液相色谱 盐酸多西环素 氟苯尼考 氟尼康 |
| DOI： |
| 投稿时间：2009-03-03修订日期：2009-03-12 |
| 基金项目: |
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| Determination the Bases of Veterinary Drug Funicon By High-Performance Liquid Chromatography |
| 李志伟,刘佳 |
| (Hebei University of Science and Technology) |
| Abstract: |
| ABSTRACT: OBJECTIVE To establish a HPLC for the simultaneous determination of doxycycline hydrochloride and florfenicol in veterinary drug funicon. METHODS The liquid Chromatography was carried out by a Hypersil-ODS2（4.6 mm×250 mm,5 μm）.The mobile phase was acetonitrile,methanol and 0.1mol•L-1 ammonium phosphate monobasic of pH 3.5(1:2:5) and the flow rate was 1mL•min-1. Funicon samples were measured with UV detector at 254 nm. RESULTS This experiment can get a better result of separation between doxycycline hydrochloride and florfenicol in funicon.The linear range of doxycycline hydrochloride was 6.25 μg•mL-1~200 μg•mL-1 and florfenicol was 6.25 μg•mL-1~250 μg•mL-1 (r=0.9999).The average recovery of doxycycline hydrochloride was 99.29%,relative standard was 0.51% (n=3). The average recovery of florfenicol was 98.87%,relative standard was 0.32% (n=3). CONCLUSION The method is simple,rapid and sensitive with good repeatability，can be used for content determination of funicon.
KEY WORDS: HPLC; doxycycline hydrochloride; florfenicol; funicon |
| Key words: HPLC doxycycline hydrochloride florfenicol funicon |
