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一株鸡传染性喉气管炎病毒流行毒株 的鉴定与致病性分析
王静文,张敏,陈玲,杨承槐,蒋桃珍
0
(中国兽医药品监察所)
摘要:
为保证鸡传染性喉气管炎疫苗效力检验的一致性和稳定性,本研究制备了一株鸡传染性喉气管炎病毒,并对其性状、纯净性、真空度、病毒含量、特异性等进行了鉴定,重点评价不同病毒含量对不同日龄鸡只的致病力。结果显示,本研究制备的鸡传染性喉气管炎病毒冻干物性状良好,无细菌、支原体和外源病毒污染,可被特异性血清完全中和,病毒含量稳定在105.1 EID50/0.2mL。该毒株致病力稳定,100EID50可使80%鸡只发病或死亡,可作为一株良好的检验用强毒,为后续疫苗评价奠定基础。
关键词:  鸡传染性喉气管炎  疫苗  效力检验  检验用强毒
DOI:
投稿时间:2022-01-10修订日期:2022-11-14
基金项目:重组动物疱疹病毒研究平台的建立 ( GY202017)
Preliminary Research and Virulence Assessment on One Virulent Strain of Infectious Laryngotracheitis Virus
,张敏,,,蒋桃珍
(China Institute of Veterinary Drug Control)
Abstract:
In order to improve the consistency and stability of potency test for infectious laryngotracheitis(ILT)vaccine, one freeze-drying virulent culture was prepared , and its characteristics, purity, vacuum degree, viral loads, and specificity were determined. Primary assessment was conducted on virulence for chicken of various age and virus detection post challenge. Results showed that this virulent strain has a good characteristic and purity, free from mycoplasma and exogenous virus contamination. It could be neutralized by specific serum and virus titers could reach 105.1EID50/0.2mL. At least 80% of unvaccinated control chicken died or showed severe clinical signs of ILT and notable macroscopic lesions at the dose of 100EID50. Studies suggest that this prepared culture could be a good candidate as an appropriate challenge virus strain.
Key words:  Chicken infectious laryngotracheitis, ILT, Vaccine, Potency test,Challenge Virus

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