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新型鸭呼肠孤病毒广东分离株σC蛋白基因序列分析
张思远,卢秀娴
0
(广州市华南农大生物药品有限公司)
摘要:
从广东湛江某肉鸭养殖场发生脚软、关节肿大为临床特征和脾脏肿大、肝脏有坏死灶为病变的樱桃谷鸭病料中分离到一株新型鸭呼肠孤病毒,命名为GD693。对新型鸭呼肠孤病毒GD693株σC蛋白基因进行 RT-PCR 扩增、克隆和测序,并与参考毒株σC蛋白基因序列进行比对分析。结果显示:GD693株与新型呼肠孤病毒(NDRV)代表毒株处于进化树的同一大分支,但却处于一个单独的分支,同属于基因2型,具有相近的遗传演化关系;与NDRV代表毒株091株、TH11 株的σC蛋白基因序列进行比较分析,存在核苷酸和氨基酸的序列改变,毒株有可能发生变异或毒力增强。
关键词:  新型鸭呼肠孤病毒  分离鉴定  σC蛋白基因  序列分析
DOI:
投稿时间:2019-08-29修订日期:2019-09-20
基金项目:
Sequence Analysis of Sigma C?of Novel Duck Reovirus Isolated
(Guangzhou South China Biological Medicine Co.Ltd)
Abstract:
A strain of novel duck reovirus (GD693) was isolated from cherry valley duck that was affected by soft feet, enlarged joints, enlarged spleen and necrotic liver lesions in a meat duck farm from Guangdong province. The sigma C protein gene of the novel duck reovirus strain was amplified, cloned and sequenced by RT-PCR, and compared with the reference strain sigma C protein gene sequence. The results showed that GD693 was in the same branch of the evolutionary tree as the novel reovirus (NDRV), but they were in a single branch, which belonged to the gene type 2 and had similar genetic evolutionary relationships.Compared with the sequence of sigma C protein gene of NDRV representative strain 091 strain and TH11 strain, there were nucleotide and amino acid sequence changes, and the strain may be mutated or virulence enhanced.
Key words:  A novel duck reovirus  isolation identification  Sigma C protein gene  sequence analysis

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